endothelial cell culture under perfusion on a polyester-toner microfluidic device - film polyester
This study proposes a cheap and easy way to produce a microfluid device that simulates blood vessels as a starting point for cell culture under perfusion, cardiovascular research, and toxicology studies.
End point analysis (i. e.
, MTT reduction and no analysis)
Use and display a microchip assembly made of polyester and Toner (PT)
Cell death or no is not induced (NO)production.
The application of oxygen plasma and fiber junction protein improved the adhesion and proliferation of vascular endothelial cells along the micro-channel.
As expected, these treatments show vascular endothelial growth factor (VEGF-A)
Concentration distribution, which is associated with adhesion and cell proliferation, thereby promoting intramination of new vascular formation devices.
Regardless of the simplicity of the device, our "veins-on-a-
The chip "analog has the potential to be a powerful tool for those who need a rapid micro-manufacturing approach for cell biology or organson-a-chip research.
In multi-cell organisms, tissues are made up of different types of cellsdimensionally (3D)
In turn, different kinds of tissues are combined to form larger functional units called organs.
In the organ micro-environment, the cell experiences a specific
Dynamic changes of organs such as chemical gradient and mechanical force (.
, Compression and fluid tension).
This force is essential for the growth, survival, response and function of cells, tissues and organs.
Therefore, in order to understand the pathology of human beings, it is extremely important to study how cells and tissues function together.
Cell culture is ubiquitous in cell biology, biochemistry, drug discovery and development, drug generation dynamics research, and tissue engineering.
The basic research of human biology and the development of therapeutic drugs usually depend on twodimensional (2D)
A single-layer cell culture system or animal model.
However, the 2D cell culture system cannot accurately reproduce the structure, function, physiology, and highly complex and dynamic 3D environment of the living tissue.
Therefore, many studies involving physiology and pathology require the use of animal models with various problems, such as: high costs, ethical issues, and, in many cases, these models are not suitable for predicting and reproducing human responses.
Therefore, due to the interaction, animal models and models are ineffective models in terms of predictive response
Species differences and/or lack of physiological-related 3D tissue environments.
Therefore, the limitations of cell culture use and existing animal models are the stimulation of the development of new alternative models that can better simulate the complex structural functions of organs.
Therefore, due to the application of Microsystem Engineering in cell culture, considerable progress is being made in this field.
Microfabrication technology enables the development of microchips that can accurately control cells, locations, functions, and respective organizational structures in highly structured cultures.
In addition, when the micro-fluid technology is combined with the micro-chip, more dynamic control of the flow and pressure of the fluid can be carried out, which can create an environment around the cells, this environment produces a variety of chemical gradients and dynamic mechanical signals that can induce cell reactions that are very similar to physiological responses
Like the environment. Organ-on-a-
The chip can simulate similar environments and subsequent similar reactions to produce realistic models of human organs of interest, which may provide the body's physiologyRelated models.
Development of organs-on-a-
Chip is a combination of micro-flow control technology and micro-engineering technology such as cell biology. Organs-on-a-
The chip reorganizes the structural arrangement of the tissue, similar to the functional complexity of the organ.
Use cell culture to produce cell micro-fluid structure
The structure and cell environment that affect the cell response. Organs-on-a-
Chips are devices that allow the study of several biological processes (
Physiology and Pathology)
It is impossible to observe using traditional cell culture systems or animal models.
From this technology, organson-a-
Chip produced various cell culture models in 3D, simulating organs such as lung, liver, kidney, cornea, neural network, and models of pathological studies such as breast cancer.
These models of organson-a-
Chip Enable organization
The tissue interface, such as epithelial/mesenchymal cells or substantial cells/endothelial cells, acts through chemical communication, nutrition, hormones, metabolites, cytokines, physical signals, and physiological fluids, more accurately indicate what happened in the living officer.
For the first time, Duffy and his colleagues described the procedures for the use of polymers to manufacture closed micro-fluid systems, which became the most popular manufacturing of micro-fluid devices, poly (
This technology allows the production of micro-devices in less than 24 hours, which indicates a significant reduction in manufacturing time compared to the production of glass microchips.
Looking for alternative materials and simpler manufacturing methods, Thompson and his colleagues reviewed polyester fiber in great detail
Carbon powder micro-manufacturing is a feasible process for producing simple and low-cost micro-fluid devices.
The PT method uses a layer of toner deposited on a polyester film that defines a microfluid channel;
In addition, Toner is used as an adhesive to seal the device through the steps of thermal lamination.
Therefore, the micro-chip of polyester Toner (PT)
It provides a very promising platform for chemical and biochemical analysis.
Using this method, several types of PT chips were created for DNA analysis, enzyme analysis of proteins, glucose and cholesterol in serum samples, and drug analysis.
Here, we report an alternative model of perfusion cell culture that simulates blood vessels, which aims to study inflammatory reactions and toxicity, thus providing better results than animal models (
Poor human prediction)
Or conventional cell cultures (non-
3D cell architecture.