Polycarbonate membrane impression cytology: evidence for fluorescein staining in normal and dry eye corneas - polycarbonate
Background/purpose corneal impression cell examination is usually performed with a mixed cellulose ester membrane and a limited stain array.
A method of using air with polycarbonate film
Dry preparations led to the discovery of fluorescent staining in the cells of patients with and without dry eye disease and membrane disease
It is not a defect caused by cell removal.
Methods impression after fluorescent installation using polycarbonate and mixed cellulose ester membrane with rapid dyeing scheme
It's as fast as Sumu and Yi red stains.
Before air staining
Fluorescent inspection of dry materials.
Results The epithelial cells of normal cornea and dry cornea retained the fluorescence of clinical infusion fluorescence.
Corneal defects caused by polycarbonate membrane cannot be explained by membrane
Induced cell clearance.
After rapid staining, the polycarbonate membrane showed less background and was easily dissolved before the coverslip was applied, but the cell yield was lower compared to the mixed cellulose membrane.
Conclusion The rapid stain can be evaluated immediately by polycarbonate membrane impression cell examination.
In normal and dry eye patients, local application of Sodium Fluorescence penetrates the corneal epithelial cells.
Cells emit fluorescence on the cell membrane. The impression-
Induced defects on the cornea are not due to cell stripping, which may represent the removal of mucus.
Funding NIH R01-
Ernst & Young 11224 and professor positions in ophthalmology for Edith and Lou Wassman.
No one declared a competitive interest.
Obtain patient consent.
Get ethical approval.
Uncommissioned source and peer review;
External peer review.