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synthesis of nm-phb (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant staphylococcus aureus - film packaging material

by:Cailong     2019-07-12
synthesis of nm-phb (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant  staphylococcus aureus  -  film packaging material
Melanin is a common dark brown photosynthetic pigment, which has a variety of and expanding applications in radio manufacturing.
Protective materials in food packaging, cosmetics and medicines.
In this study, the melanin production of P.
Isolated from sea sponge teyrina Citi rna using one-
Factors of one experiment, and the chemical properties and stability were characterized.
Nuclear nano-protein after ultrasonic treatment (Nm)
Particles were formed and evaluated for antibacterial and antioxidant properties.
Combination of nano-composite film preparation (% w/v)
Polyacetate-nanomelanin (PHB:Nm)
Mixed with 1% glycerin.
The Nm was found to be spherical, with a diameter of 100-140 nm, and has strong antibacterial activity against both Gram-positive and gram-negative bacteria. The Nm-
The PHB nano-composite film is uniform, smooth, crack-free and flexible.
X-ray diffraction and DSC data show that the film is crystalline in nature and has a thermal stability of up to 281. 87u2009°C.
This study represents the first report on Nm synthesis and Nm-manufacturing
PHB nano-composite film that has a strong protective effect on multi-drug resistant Staphylococcus aureus. Thus this Nm-
PHB nano-composite films can be used as food packaging materials by protecting food from oxidation and bacterial contamination.
Melanin is an important ubiquitous pigment in biology and exists in all the kingdoms of life.
They usually provide protection against environmental pressure, such as solar UV rays and energy transfer.
They are complex and diverse polyblack or brown pigments formed by oxidation polymerization of phenol and/or inert compounds or by polyketide pathway.
The tyrosinase enzyme participates in the most famous melanin Biological synthesis pathway by producing iron-chromium (red)
Or eumelanins by oxidation of tyroine to l-3,4-
Dopamine (l-DOPA)
And doraquone.
Production of melanin and activity of cheese tyrosinase in soil bacteria (eg and sp)
As we all know, they play a role in the symbiotic relationship with plants.
However, the production of melanin is also important in the pathogenic forces of several bacteria and fungi.
It can directly affect the host's immune response to infection by increasing the resistance of pathogens to the host's antibacterial mechanism.
Subsequently, both the Melanin biosynthetic pathway and the melanin itself are potential targets for the discovery of antimicrobial agents.
Melanin is a negatively charged, hydrophobic, non-crystalline and high-molecular-weight compound that is resistant to chemical degradation of acids and antioxidants.
Synthesis of melanin can be prepared by chemical oxidation of dopamine or by enzyme-promoting oxidation.
The size of the synthetic melanin particles is uneven and does not dissolve in water, so the size-
There is no controlled synthesis method for synthetic melanin particles.
Melanin has a variety of commercial uses, including the preparation of cosmetics as a sunscreen factor (SPF)
They function by absorbing radiation and preventing light-induced skin damage.
By preventing cell damage caused by ultraviolet rays (UV)
B. It also helps to prevent certain skin diseases.
They also found applications as antioxidants as anti-cancer and anti-cancer
Inflammatory drugs and immune stimulation drugs.
They are also used in semiconductors and biology.
Application of electronic products, as well as sunglasses lenses, paint and varnish in polarized.
Because black is nice.
It is well known that the melanin binding membrane can protect cells from ionising radiation
Protective materials.
However, the melanin film is usually very fragile, and in order to increase its durability, it is usually stabilized with plasticizer such as glycerin, organic solvent or water.
The synthetic melanin film was prepared by silica coating.
High-quality melanin films have important electronic applications that can be prepared by oxidation of dopamine, but the processes available are very time-consuming and often produce uneven particle morphology.
The main purpose of food packaging materials is to prevent oxidation, microbial corruption, radiation and oxidation processes.
To replace synthetic plastics in food packaging, based on various bio-plastics and microbial products, new sources of edible/biodegradable food packaging materials are being developed. PHB (
Poly-dinate
Due to its medical, food packaging and resistance
Adhesive applications.
Although PHB has a high thermal stability and crystalline degree, it is a challenging process to process it into a flexible film.
However, the use of such as heat treatment, co-
By polymerization mixing and adding plasticizer, PHB can be made into flexible film with high heat and mechanical properties.
Polymers such as PHB have crystalline phase and have good mechanical properties and penetration properties, thus forming suitable and safe packaging materials.
PHB mixed with PLA (
PLA)
Due to its high biodegradable capability, it has been applied in various food packaging applications.
Nano-systems are also becoming more popular in efficient drug and chemotherapy delivery, suggesting reduced side effects.
Bacteria isolated from marine sources from seaweed and marine sediment have previously been reported as melanin producers.
As we all know, sponges are the source of complex microbial communities including bacteria, fungi and ancient bacteria with ecological and biotechnology importance.
Marine Sponge-related microorganisms account for 40% of the total sponge biomass and have been known to produce new antimicrobial agents, biologically active compounds, anti-fouling agents, and many enzymes of industrial interest.
Sponge-related actinobactium MSA10 has also been developed for the production of melanin previously.
Here we report the production of melanin, the optimization of melanin production, and the synthesis of nano-bitter (Nm)
And manufacturing of Nm-
PHB nano-composite film was prepared by mixing melanin with melanin.
The aim is to produce a film with increased durability with the potential to prevent microbial colonization and biofilm formation.
For this, Nm-
The thermal stability, structure and morphology properties of PHB nano-composite films and their resistance
Effects on multiple infections
Drug resistance (MDRSA).
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